DETERMINAÇÃO DA NATUREZA DO ÁCIDO NUCLÉICO DE BACTERIÓFAGOS QUE INFECTAM Xanthomonas perforans ASSOCIADAS A MANCHA BACTERIANA DO TOMATEIRO

Abstract

Bacteriophages are used as biological control agents for various phytobacterial diseases. For tomato bacterial spot, its use has been shown to be successful in controlling it when compared to chemical control. Due to the enormous diversity of bacteriophages, the nature of the nucleic acid is one of the criteria for classification into Order and Family, as well as morphology. For this reason, the objective of this work was to characterize the nature of the genetic material of bacteriophages from the collection of the IF Goiano Campus Morrinhos that infect Xanthomonas perforans, which causes bacterial leaf spot in tomato. Nucleic acid extraction of six bacteriophage isolates was performed and 3 aliquots of 10 µl were reserved from each isolate. The first aliquot received the RNAse treatment, the second was treated with DNase, and the third was the control and received no treatment. All treatments were performed as indicated by the enzyme manufacturer. After treatment, aliquots of nucleic acid were subjected to electrophoresis in a 1% agarose gel at 100 V for 40 min. Then, the gel was stained with ethidium bromide, placed in a transilluminator with UV light and the result was observed through the image obtained with a photodocumenter. It was possible to determine that all bacteriophages, F1, F2, F3, F4, F5 and F6, present deoxyribonucleic acid (DNA) as genetic material.