MULTIPLICAÇÃO IN VITRO DE BROMÉLIAS AECHMEA AQUILEGA E BROMELIA BALANSAE

Abstract

The objective was to evaluate the in vitro multiplication of A. aquilega and B. balansae as afunction of combinations of naphthalene acetic acid (ANA) and 6-benzylaminopurine (BAP). The experiment was carried out in a 2x3x4 factorial, with two species of bromeliads (B. balansae and B. aquilega), three levels of ANA (0.0; 0.5 and 1.0 mg.L-1 ) and four levels of BAP (0.0; 1.5; 3.0 and 6.0 mg.L-1), in a completely randomized design, with three replications and six seedlings per experimental unit. Seeds were used to implant the cultures in vitro. After seed germination, in a test tube containing MS medium, with half the concentration of salts, the seedlings were transferred to plastic containers, containing 60 ml of MS medium, supplemented with the proposed ANA and BAP concentrations. The interaction between ANA and BAP influenced only the number of shoots. The interaction between genotypes and BAP influenced the sprouting. The use of cytokinin was decisive for the proliferation of shoots. There was a difference between the bromeliads in terms of sensitivity to the growth regulators studied. A. aquilega showed higher efficiency of sprouting in medium with 5.05 mg.L-1 of BAP, while B. balansae did not reach maximum sprouting with 6.0 mg L-1 BAP. The presence of BAP reduced rooting. There was a difference in the fresh and dry mass of the genotypes. B. balansae had the highest fresh weight. Both species demonstrated ease in the proliferation of shoots, as well as in vitro rooting. It is recommended to use cytokinin for the multiplication of the two species of bromeliads studied and to obtain seedlings with potential for acclimatization, only the MS medium