QUALIDADE FISIOLÓGICA DE SEMENTES Dipteryx alata vog. ARMAZENADAS: TESTE DE TETRAZÓLIO E IMAGEM RADIOGRÁFICA

Abstract

The uncontrolled increase in agroindustrial activities and human occupation of the Cerrado domain has contributed to the loss of habitats of various plant species, among they baru (Dipteryx alata). In this scenario, test standardization that determines the quality of physiological, seed longevity and vigor are crucial to conservation of plant species. Thus, the objective of this study was to standardize the methodology of tetrazolium test on baru seeds and to evaluate the physiological quality of seeds stored for 12 months at 10 and 20 ° C, using X-ray images as the main damage detection tool over the period. The outline The experimental design was completely randomized with 4 repetitions. For test 1: definition of tetrazolium test methodology in baru seeds were tested three concentrations of tetrazolium salt, 0.05, 0.075, 0.10% and two pre-imbibition times, 24 and 36h. For the rehearsal 2: baru seed storage were evaluated 5 storage times, 0, 3, 6, 9, 12 months and two temperatures, 10 and 20 ° C. The seeds of Baru remained in this viable for 12 months of storage, but with small reduction in from nine months. The methodology applied for the tetrazolium test was efficient to determine internal damage to Baru seeds. The pre-soak time for the test of Recommended tetrazolium is 24 hours. Throughout storage periods no observed changes in seed morphology and radiographic images obtained by the test X-rays, regardless of the temperature evaluated. The intensity and time of 30 kv and 45 seconds, respectively, made possible the precise visualization of the structures internal baru seeds by the X-ray test.