USO DE LINHAGENS DE Trichoderma harzianum SUPEREXPRESSORAS DE QUITINASE NO PARASITISMO DE Pratylenchus brachyurus

Abstract

ABSTRACT: Phytonematoide Pratylenchus brachyurus has as characteristics a geographical distribution, high degree of polyphagia, aggressiveness and pathogenicity. And due to losses in several crops produced in Brazil. And thinking about its use of nematicides becomes an alternative alternative, especially the biological nematicides especially based on Trichoderma harzianum, and each phase of the fungus presents the differentiated for the management of nematodes. Those that come with higher expression of enzymes such as chitin may present higher parasitism and stand out as too many. Overheating of T. harzianum non-parasitic chitinase superexpressors of P. brachyurus J2 juveniles. This trial had 6 treatments, namely T1- T. harzianum wild strain, T2- T. harzianum Exo strain. 3.1.2 Chitinase superexpressor, T3- T. harzianum strain Exo. 6.2 chitinase superexpressor, T4-T. harzianum strain Exo. 14 chitinase superexpressor, T5-Ecotrich © (T. harzianum IBLF 006) and T6- Trichodermil © (T. harzianum CEPA ESALQ 1306), with 5 replications, in a completely randomized design. T. harzianum parasitism on P. brachyurus J2 juveniles in vitro was evaluated 5 days after inoculation. The means were compared by the 5% Kruskal Wallis test. T. harzianum IBLF 006became the most efficient non-parasitic P. brachyurus, while T. harzianum CEPA ESALQ 1306should appear the worst result. Already as overexpressing and wild strains were statistically. Thus, it is concluded that chitinase overexpressing T. harzianum strains present parasitisms on P. brachyurus J2 juveniles.