COMPOSIÇÃO QUÍMICA E ATIVIDADES BIOLÓGICAS DOS ÓLEOS ESSENCIAIS DAS FOLHAS, FRUTOS VERDES E MADUROS DE Murraya paniculata (L) Jack.

Abstract

The Murraya Paniculata (L) Jack species is popularly known as myrtle scent and has a great therapeutic potential described in the literature. Its main biological activities are conferred by its terpenes presence in their chemical composition. The objective of this study was to determine the chemical composition and biological activities of the essential oils extracted from the leaves, green and ripe fruits of M. paniculata. The SOs was obtained by the conventional hydrodistillation technique in Clevenger type apparatus. And the identification of the compounds present in the OEs was performed by gas chromatography coupled to mass spectrometry (GC-MS). The anticariogenic activity of fruit (Green and Ripe) OEs was evaluated against oral pathogens: Streptococcus mutans, S. mitis, S. sanguinis, S. sobrinus and S. salivarius. As a result, the SOEs exhibited moderate activity with MIC values between 100 and 400 µg / mL. Regarding antimicobacterial activity, SOEs were active against Mycobacterium kansasii (MIC = 250 µg / mL), moderately active against M. tuberculosis (MIC = 500 µg / mL) and inactive against M. avium (MIC = 2000 µg / ml). The leaf EO antifungal activity was investigated by the disk diffusion methodology against the fungi Sclerotinia sclerotiorum (Lib.). Different concentrations of diluted EO in DMSO were used. Thus, it was observed that at the 300 µL dose of EO showed 91.2% inhibition of fungal mycelial growth. For antioxidant activity, the method of sequestering capacity of the 2,2-diphenyl-1-picrilhydrazine (DPPH) radical was used. The assay showed IC50 values = 932.55 µg / mL 1123.72 µg / mL and 716.72 µg / mL for leaf and fruit OEs (Ripe and Green), respectively. Finally, to evaluate the leishmanicidal activity, the promastigote forms of Leishmania amazonenses were used. As a result, it was observed that the leaf OEs showed high leishmanicidal activity, with IC50 = 7.33 ± 2.07 μg / mL. Meanwhile, the fruit OEs (Ripe and Green) presented IC50 values = 13.04 ± 1.64 and 30.77 ± 2.07 μg / mL, respectively. This paper describes for the first time the chemical composition as well as the biological activities of different parts of Murraya Paniculata (L) Jack essential oil collected in Brazil.