USO DA ESPECTROSCOPIA DE ABSORÇÃO AO INFRAVERMELHO NO ESTUDO DE RAÇÕES PARA CÃES

Abstract

The present work sought the evaluation and study of Peroxidation occurring in Lipids, such as bulk feed cities of Nova Glória and Ceres, both in the interior of the state of Goiás. A total of 21 dog food samples were purchased in bulk and subjected to Fourier Transform (FTIR) Medium Infrared Absorption Spectroscopy. The ready shares were submitted to an evaluation in the medium infrared region, from 4000 to 400 cm-1 were compacted with a pressure of 80 N for 10 minutes, whose constitution of 1 mg of the sample to 80 mg of KBr and dried in greenhouse for 30 minutes at 110 °C. The readings in the Medium Infrared Absorption Spectroscopy equipment were at a 4 cm-1 resolution with the use of 100 scans to obtain the spectrum. All sample readings were in triplicate, according to the qualitative analysis, the presence of some functional groups was observed, such as: What has the wavelength 720 cm-1, a - (CH2)n - group which is the methylene also at wavelengths at 1300-1050 cm-1 the CO composition of saturated esters can be observed, in the region of 1470-1430 cm-1 may be associated with the CH2 as a chain deformation in 1750-1740 cm-1 can be located a clearly associated peak C=O of esters in the aliphatic 2960-2850 CH region and at the wavelength of the 1715 cm-1 region is inferred the presence of a carbonyl which may means the presence of malonaldehyde which is a lipid peroxidation product compound. The data were treated with pre - treatment and pre - processing methods, such as the first derivative and average data centering. Finally, the data were submitted to Principal Component Analysis (PCA). The first PC that was PC1 was able to explain (55.2%) and PC2 that explained (25.3%) together they were able to explain the majority of the data, which was 80.5%. The graph with respect to PC1 X PC2 noticed the presence of 3 clusters, where it showed the chemical similarity between the samples.