IDENTIFICAÇÃO DE SNPs EM GENES DE AVIRULÊNCIA DE Magnaporthe oryzae

Abstract

The main disease of rice culture is blast, caused by the fungus Magnaporthe oryzae, one of the most destructive diseases, which causes significant losses in rice production. The fungus has high variability and is organized in populations that are composed of pathotypes or physiological races with distinct virulence characteristics. The objective of this work was to perform the genomic analysis of Brazilian M. oryzae isolates from complete genome sequencing. Isolated from the collection of microorganisms from Embrapa Arroz e Feijão collected in six different states (MG, MT, SC, RS, TO and MA), from upland and irrigated cultivars. The most virulent isolates were selected by inoculation in the differentiating series of Embrapa Arroz e Feijão, MGa87, MTa91, SCi97, RSi59, TOi67, MAi68. The isolates were grown in BDA medium for DNA extraction and for complete genome sequencing via Illumina® technology. Altogether, 80,468 SNP-type markers were identified, with 42 SNPs linked to avirulence genes. Among the avirulence genes, one SNP was identified for Avr-Pizt, two SNPs for AvrCO39, Avr-Pii, Avr-Pik / k / m and AvrPib; three SNPs for Avr Pi54 and four Avr-Pita 1. An average of 11,800 contigs was obtained from the assembled genomes, with coverage ranging from 43x to 44x and an average GC content of 51%. With these data, we hope in the near future to design primers for the validation of these SNPS markers. With the results of the genomes assembled, we believe they are suitable for further studies, such as: genomic annotation and genomic prediction.