ESTUDO BIOTECNOLÓGICO E PERFIL QUÍMICO DE PLÂNTULAS MICROPROPAGADAS DE Hyptis marrubioides Epling

Abstract

Hyptis marrubioides is a Brazilian Cerrado plant and popular name described as mint field, which presents great morphological diversity and antioxidant potential. The investigation of this specie, using tissue culture techniques through abiotic elicitation becomes interesting to promote increased production of healthy plants with high levels of substances of interest. Therefore, the objective of this study was to evaluate the effect of abiotic elicitation in seedlings of Hyptis marrubioides in vitro and explore its chemical and biological profile. There were using as elicitors different wavelengths of light in the white, blue, green, red and yellow spectral, plus salicylic acid and silver nitrate in concentrations of 30 uM and 60 into two separate crops. Growth parameters, shoot length, number of leaves, fresh and dry weight were investigated, as well as rutin content by HPLC-DAD. The average length of shoots and average number of leaves showed significant differences for the period of 30 days of exposure to light, where to the red light the length reached 15.46 cm and the white light with 9.20 cm. Greater abundance of dry mass was observed in the 30 days period for the seedlings grown in red light with 26% increase compared to the control. The seedlings grown under yellow, red and green light in the twenty days period had similar average values of 0.10 mg g -1 DM. However the treatment with abiotic elicitors were similar among them for growth parameter, while rutin content was hight to treatment with SA at a concentration of 30 uM in the second method within 6 days, with 4.80 mg g -1 DM while the control with 0.27 mg g -1 DM (17 times more than the control). The ethyl acetate extrac were analyzed by CG-MS, respectively, allowing the identification of the two compounds aromadendrenepoxide and (3 beta, 24S ) stigmast-5-on-3LO. The schistosomicide potential of crude extract of these two types of crop was evaluated in vitro against adults worms of Schistosoma mansoni. The results indicated that the extract field (ex vitro), was the most active among both crude extracts tested.