OBTENÇÃO DE PADRÕES ANALÍTICOS DE CAROTENOIDES POR CROMATOGRAFIA EM COLUNA E VALIDAÇÃO DO MÉTODO PELO GRAU DE PUREZA EM UV/VIS

Abstract

This study investigated the potential of murici pulp (Byrsonima crassifolia, Malpighiaceae) as a source of carotenoids for analytical standards. The pulp was obtained from a certified supplier and processed through cold acetone extraction (4°C), followed by filtration and liquid-liquid partitioning with chilled petroleum ether. The carotenoids were purified by column chromatography (CC) using silica gel as the stationary phase and petroleum ether as the eluent. UV-Vis spectrophotometry analysis (350-700 nm) revealed characteristic peaks at 446 and 473 nm, consistent with β-carotene standards (450 and 470 nm), confirming the presence of these compounds. The CC technique proved efficient for pigment isolation with satisfactory recovery. Results indicate that murici extracts have suitable characteristics for analytical standard production, offering advantages such as accessible raw material and a simplified extraction method. Furthermore, the spectrophotometric similarity with commercial standards suggests potential applications in food and supplement quality control. This work contributes to the valorization of native fruits as sustainable sources of bioactive compounds, opening new perspectives for natural products chemistry research and analytical method development.