SENSIBILIDADE DE ISOLADOS DE Trichoderma spp. COM BASE EM ESTRESSE SALINO E SORBITOL

Abstract

Trichoderma spp. species play a crucial role in agriculture and soil ecology, acting in biological control, stimulating plant growth and contributing to the biodegradation of pollutants. This study evaluated the sensitivity and classification of Trichoderma spp. isolates under salt and osmotic stress. A total of 23 isolates from different regions (GO, DF and PR) were analyzed, cultivated in potato-dextrose-agar (BDA) medium supplemented with NaCl and sorbitol. The experiment was conducted in a completely randomized design, with two factors: isolated (23 treatments) and type of stress (three treatments), totaling 138 experimental units, considering that two replications were performed per treatment. Colony diameter (mm) was evaluated daily for seven days, allowing the calculation of the area under the mycelial growth curve (AACPCM) and mycelial growth rate (MCT, mm day-1). The results showed significant variations among the isolates, reflecting the genetic and physiological diversity of the species. Isolates such as IF84 and IF42 showed rapid growth under the stress types, presenting themselves as biotechnological potential for biological control under conditions of salinity and osmotic stress, while IF136a and IF136b grew less, and can be explored in symbiotic interactions. Growth was reduced in the media supplemented with NaCl and sorbitol compared to the control, but without significant differences between the types of stress. The results reinforce the potential for selecting specific lines for biological management under adverse conditions, reinforcing the applicability of Trichoderma spp. in sustainable strategies.