PADRONIZAÇÃO E VALIDAÇÃO DE MARCADORES MOLECULARES PARA ESTUDOS DE DIVERSIDADE GENÉTICA EM Mauritia flexuosa L.f.

Abstract

The present study aimed to standardize and validate microsatellite markers for the analysis of the genetic diversity of the buriti palm (Mauritia flexuosa L. f.). For this purpose, a set of microsatellite markers was selected based on genetic databases and relevant literature. Buriti samples were collected from different geographical areas to ensure the representativeness of genetic diversity. DNA extraction was performed using the CTAB method (2X) with modifications for buriti trees, followed by amplification of microsatellite markers through PCR. The amplified products were analyzed using agarose gel electrophoresis to verify the presence of the expected fragments. The results were promising, as the microsatellite markers revealed distinct genetic variations among the sampled buriti populations, providing insights into the genetic structure of the populations and their relationship with environmental and historical factors linked to fragmentation. These implications pave the way for a deeper understanding of the genetic diversity of buriti palms in Goiás and its implications for the ecology and conservation of the species.