TAXA DE RECUPERAÇÃO DE OÓCITOS DE DOADORAS HOLANDESAS

Abstract

Brazil has one of the largest comercial cattle herds in the world. The in vitro production of embryos demonstrates a high importance in the to the reproductive techniques used for Brazilian dairy production, especially for females of the Gir and Holstein breeds. This study aimed to evaluate the performance of Holstein breeders as oocyte donors after ultrasoundguided follicular aspiration (ovum pick up – OPU), by rates of oocyte retrieval and in vitro maturarion (IVM). A data collection of follicular aspirations guided by ultrasound and IVM was carried out of oocytes from Holstein donors during the year 2015. 272 were accompanied Ultrasound-guided folicular aspirations at 15-day intervals. The characteristics analyzed were: number of viable and non-viable total oocytes, number of grade I, II, III oocytes and% of viable oocytes after IVM. For statistical analysis descriptive statistic were performed for the characheristics analyzed. the assessment of the significance of the effects (months of the year and property) were submitted Analysis of Variance using the PROC GLM of the Statistical Analysis System. For comparisons of means was performed Tukey’s test at a level of significance 5% (p>0,05). Were observed after the mean OPU of: 24,88 ± 13,82 for total oocytes; 17,51 ± 10,85 for viable; ; 2,28 ± 3,26 for grade 1 and; 6,33 ± 5,82 for grade 2 and; 8,89 ± 7,18 for and grade 3; 7,39 ± 4,52 for non-viable oocytes; e 21,47 ± 12,31 for% viable after the IVM. There was a significant difference between seasons for recovery rates of grades I, II and non-viable oocytes. When analyzing the quality of oocytes aspirated by season of the year, a greater number of grade I oocytes recovered in autumh and spring was obserbed. However, in the summer season, they had the lowest quality index and the lowest number of oocytes. It was concluded that the donors of the Holstein breed of the presente study showed satisfactory oocyte retrieval rates.