PERFORMANCE DE DOADORAS JERSEY À ASPIRAÇÃO FOLICULAR GUIADA POR ULTRASSOM E FERTILIZAÇÃO IN VITRO

Abstract

This study aimed to evaluate the performance of Jersey donors after follicular aspiration guided by ultrasound (ovum pick up - OPU), by the oocyte recovery rates, and in vitro fertilization (IVF), by the rates of embryos produced. A survey of OPU and IVF data from donors of the Jersey breed was carried out during 2013. Data from 113 follicular aspirations from 22 donors were used. For in vitro fertilizations, commercial doses of sexed semen were used. Data were collected regarding: number of total oocytes after OPU, number of viable and non-viable oocytes, number of oocytes grade I, II, III, number of viable oocytes after in vitro maturation (IVM), number of viable ones after cleavage, embryos produced after in vitro culture (IVC), embryos transferred to recipients, positive recipient pregnancy diagnosis at 30 days and quantity of pregnancy at 60 days. For statistical analysis, descriptive statistics was performed for the analyzed characteristics. The evaluation of the significance of the effects (season and bulls) was performed by Analysis of Variance, using the PROC GLM procedure of the Statistical Analysis System. To compare the means, the Tukey test was performed, at a significance level of 5%. The following averages were observed after the OPU, by donor: 17.608.45 total oocytes; 3.162.61 non-viable oocytes; 14.437.67 viable oocytes; 2.322.93 grade I oocytes; 7.024.20 grade II oocytes; 5.073.78 grade III oocytes; 9.215.25 viable oocytes after IVC. After IVF, averages of: 3.353.07 embryos produced were observed; and 2.622.74 embryos transferred. The average total Jersey oocytes in this study is considered satisfactory. The influence of the seasons on the analyzed characteristics was significant (p> 0.05). For bulls, however, a difference was found in this study for the rates of cleavage and embryos produced. It is concluded that the oocyte recovery rates from Jersey donors have shown satisfactory results, with significant variation between the seasons, with the winter season having the best rates. The bull factor was also relevant for embryo rates.