SÍNTESE DE NANOPARTÍCULAS DE PRATA E AVALIAÇÃO DA TOXICIDADE EM CARAMUJOS

Abstract

With the increasing interest of the scientific community in nanotechnology and nanoscience, there is an interest in the search for alternative routes of safer syntheses with less impact on the environment. Part of this interest is due to the numerous applications given to metallic nanoparticles, such as sensor construction, microelectronics, catalysis, bactericidal action, drugs, among others, due to their high optical, electronic, magnetic and catalytic properties. The green synthesis of NPMs aims to use biological organisms, or parts of them (such as organs, tissues, cells or biomolecules), as substitutes for toxic chemical reagents in the synthesis reactions of nanocomposites, presenting itself as a sustainable alternative. This work aimed to report different synthesis routes, including the green synthesis of silver nanoparticles (AgNPs), with extracts from plants of the Cerrado Biome, and to test their toxicity in snails of the species Biomphalaria glabrata, vector of the parasite Schistosoma mansoni, which causes schistosomiasis. The characterization of AgNPs combined with extracts was performed by spectroscopy in the UV-Visible region; the functional groups were analyzed by Infrared Spectroscopy with Fourier Transform (IVTF); particle size was observed by transmission electron microscopy (MET); The surface charge of the nanoparticles was analyzed in a Zeta Sizer Nano Z-S device at different time intervals. The assessment of the molluscicide effect of AgNPs with plant extract from Cerrado plants was carried out in partnership with the Federal University of Goiás. The AgNPs showed maximum absorbance around 400 ηm. Surface loads ranged from -54.7 mV to -29.5 mV. Only the sample of silver nanoparticles with the borohydride reducer promoted 100% mortality and for the syntheses using extracts the ones that showed mortality were the AgNP combined with water bleeding and cashew. The synthesis process showed high reproducibility, reached the desired scale, and it can be used as a molluscicide, presenting 100% of the mortality of exposed snails.