TENDÊNCIAS DA LITERATURA CIENTÍFICA SOBRE USO DE MARCADORES MOLECULARES EM ESTUDOS DE DIVERSIDADE GENÉTICA DE PEIXES

Abstract

Genetic diversity can be defined as any measurement that quantifies the magnitude of genetic variability within a population. It isconsidered an important tool for the evaluation, conservation and management of species. However, to evaluate genetic diversity, it is necessary to access this diversity. Genetic markers are tools widely used for this purpose in studies related to the ichthyofauna, given the economic and ecological importance of these animals. Then, the aim of study was to analyze the patterns and trends of scientific publications on genetic diversity in fish populations using five classes of molecular markers (Alloenzymes, RAPDs, SSRs, ISSRs, SNPs and their derivatives), in order to verify the number of articles published in the area (since the first publication up to the year 2018), as well as to monitor the temporal variation on the number of articles, type of journals, researchers' nationality and impacts of these publications in terms of citations. To this end, a bibliographic survey was performed on the Web site of Thomson ISI Web of Science, using the key words "Microsatellite * AND fish AND genetic diversity; Alloenzyme * AND fish AND genetic diversity; RAPD * AND fish AND genetic diversity; ISSRs * AND fish AND genetic diversity; SNPs * AND fish AND genetic diversity ", in the abovementioned years. Then, different evaluation approaches were done on the articles, such as: analysis of the time trend, journal, citation profile, study nationality and collaboration. As a result, there wasdetected a significant increase in the number of studies related to fishes genetic diversity in the period from 1992 to 2018. Of these, 999 publications included the use of SSR markers, 123 publications with RAPD and, subsequently, Alloenzymes, with 61 publications, SNPs with 40 publications and ISSR with 7 publications. It was found a total of 215 different journals that published articles using the Microsatellite markers, followed by 86 different journals for the RAPD marker, 38 journals for the marker with Alloenzymes, 27 different journals for the marker SNPs and 7 journals with the ISSRs markers. It was observed a certain preference of the magazine's choice for the publications with the Microsatellites, RAPD and Alloenzymes. Concerning to the nationality of the publications, in terms of the nationality of the host institution of the corresponding author, a predominance of North American studies followed by Europe was observed. The results of the citations analysis showed a poor relation between citation and the number of authors of the paper. However, it was found a strong relationship between the citation average and the publication time: the longer the paper is available, more citations it has. Regarding the relationship between pages number and the citations average it was found a moderate relation, indicating that dense works are more accepted by the scientific community. In conclusion, these results demonstrate xiv the relevance of this topic and it is awaited that the number of papers using molecular markers approaches, such as Microsatellites and SNPs which present considerable advantages. Moreover, it is hoped that the developing countries will be able to allocate more funds in researches on genetic diversity of fishes, as they have economic and ecological relevance.