OCORRÊNCIA E IDENTIDADE MOLECULAR DO CARVÃO-DO-CAPIM-AMARGOSO (Digitaria insularis) UTILIZANDO REGIÕES ITS do rDNA E ß-TUBULINA

Abstract

Coal-causing fungi affecting weeds are poorly studied. Given the immense focus and recognition of plant pathogen interactions on plants cultivated, even though it is an important source of phytopathogen survival. The goal the aim of this work was to identify, diagnose and sequence conserved regions of the causative agent from the bitter grass. Samples of bitter grass showing symptoms of whip and collapse of the main leaf were analyzed. Semipermanent slides were prepared, for morphological, morphometric characterization and macro and microphotographic records. Ustyllospores were collected for DNA extraction, amplification using ITS-1 primers. [TCCGTAGGTGAACCTGCGG], ITS-4 [TCCTCCGCTTATTGATATGC], Bt2a [GGTAACCAAATCGGTGCTGCTTTC] and Bt2b [ACCCTCAGTGTAGTGACCCTTGGC] and sequencing. The sera presented peridium composed of fungal, filiform cells. Or thin, the sometimes clustered spherical ustilospores of diameters 8.4- (6.4) -3.1 μm, dimorphic, with no sterile cells between spore balls. The sequences were compared to those deposited in the Bank gene of the National Center for Biotechnology Information’s”. The ITS region produced 700 bp amplicons, and the gene that amplifies the region Preserved b-tubulin produced a 520-710 bp amplicons. Morphological analysis and phylogenetic analysis pointed out that the isolate detected in the municipality of Urutaí, GO, as belonging to Sporisorium panici-leucophaei (Ustilaginales, Ustilaginaceae) species only recorded in Oceania, Southern Brazil and the African continent to the present.