Viabilidade de embriões bovinos produzidos in vitro com adição de melatonina ao meio de transporte

Abstract

The use of the antioxidant melatonin to the culture medium, can provide improvement in the quality of the embryos, favoring their development. In this context, the objective was to evaluate the effect of adding melatonin to the medium of transport on the development of bovine embryos, fresh, produced in vitro, kept at 36°C for 6, 9 or 12 hours. In vitro produced bovine embryos were packed in 0.25mL straws in maintenance medium containing or not the antioxidant melatonin 10-9M to the SOFaa transport medium and kept in embryo transporter at 36°C for 6, 9 or 12 hours. After development, the embryos were evaluated and subsequently recultivated in CO2 incubator medium at 38°C. The evaluations were repeated after 24, 48, and 72 hours after the embryos were packed in recultivation. The percentage of embryos that maintained the developmental stage (MANT), developed (DES), degenerated (DEG) and hatched (ECLO) were evaluated. For statistical analysis, the Q-Square Test (p≤0,05) was used, using R program version 3.5.0. In the evaluation after 24 hours a lower rate of DEG was observed in the treatment without melatonin maintained for 12 hours in maintenance medium (Control 12). With 72 hours no difference was observed between treatments in any of the evaluated parameters. It was concluded that the antioxidant melatonin 10-9M in transport medium had no beneficial effect on the viability of in vitro produced embryos maintained for up to 12 hours in HSOfaa