PROSPECÇÃO DE MICRO-ORGANISMOS PRODUTORES DE ENZIMAS A PARTIR DA MICROBIOTA ENDOFÍTICA RADICULAR E RIZOSFÉRICA DE Hymenaea courbaril AMOSTRADA EM ECOSSISTEMA DE PANTANAL E CERRADO

Abstract

The Cerrado and the Pantanal are very biodiverse but abiotically very distinct biomes. Nevertheless, Hymenaea courbaril, popularly known as Jatoba, is widely distributed throughout these two biomes. It is a plant popularly known for its various uses, especially for medicinal properties and its use in the recovery of degraded areas, however, little is known about the identity and functional role of rhizospheric and endophytic microbiota of this plant. Due to the significance of this species it is necessary to obtain data for its conservation and valorization. In this context, this research aimed to evaluate the potential for enzymatic synthesis of the microbiota sampled in the rhizosphere and roots of Hymenaea courbaril sampled in the Cerrado and Pantanal biomes. Twenty fungal isolates and twenty bacterial isolates were evaluated. The potential for enzymatic synthesis was evaluated in culture media containing substrates for enzymatic reaction of amylases, proteases and lipases. Among the most prominent fungal isolates are FSCR6 and FSCR 8, with enzymatic indices of 21 and 17mm for amylase and 15 and 18mm for protease respectively; and among the most prominent bacterial isolates are BSCE8 and BSCE7, with enzymatic indices of 10 and 9mm for amylase and 5 and 4mm for protease respectively, however lipase synthesis was null, and the Cerrado microbiota was more efficient in this regard. Isolated rhizospheric microorganisms were also more efficient than endophytic microorganisms.