PRODUÇÃO DE SOLASODINA ATRAVÉS DO CULTIVO IN VITRO DE PLÂNTULAS E CALOS DE Solanum aculeatissimum Jacq. (JUÁ BRAVO)

Abstract

Belonging to the family Solanaceae species the Solanum aculeatissimum Jacq. has severe groups of alkaloids amoung several plants that accumulate steroidal alkaloids. The metabolite solasodine present in leaves, stems and fruits of this species has been reported as a valuable precursor steroid as a complementary source of commercial synthesis of various steroid medications, it may be obtained mainly from several plants of Solanum genus. This study aimed to produce solasodine by in vitro cultivation of callus and seedlings of S. aculeatissimum as a source of raw material in the production of steroid medicines, assessing the effect of different conditions on growth, metabolism and histology of callus obtained. The influence of different light spectra [White (300- 750 nm), blue (400-490 nm), green (490-560 nm), yellow (560-590 nm) and red (600- 700 nm)] on the growth and production of solasodine in seedlings and callus was evaluated. It follows that the production process of solasodine metabolite by cultivation in vitro of callus and seedlings S. aculeatissimum Jacq., can be influenced by light exposure and different kinetin doses. In the assessment it was possible to optimize the metabolite production and its influence on growth and immuno histochemistry in vitro, compared to the amounts obtained in the tissues of leaves and fruits field. This demonstrates that the biotechnological techniques directed to this species could serve as a source of future data and thus be a source of raw material in the production of steroid drugs.