COMPORTAMENTO ELETROQUÍMICO E ELETROPOLIMERIZAÇÃO DO ÁCIDO 4-HIDROXIFENILACÉTICO (4 HFA) NO ELETRODO DE CARBONO VÍTREO

Abstract

The electrochemical biosensor is a tool that seeks analytical information through biological recognition. A part of this equipment, it is common for the appliances used, to undergo a type of consecration known as electropolymerization that makes it possible to form stable polymeric films on the surface of the working appliances. In this work, electropolymerization was performed by cyclic voltammetry using an electrochemical cell with three electrodes, glassy carbon (CV) as working electrode, platinum wire as counter electrode, and silver/silver chloride as reference electrode. With this, experiments were carried out to build the CV electrode modified with 4-HFA aiming at a future biochemical application as a platform for the development of canine visceral electrochemical sensor. Studies of the electrochemical behavior in K3Fe(CN)6/K4Fe(CN)6, the conditioning of the electrodes in HClO4 before and after the modification and the influence of the electropolymerization of 4-HFA at different pH, concentrates and there is no number of cycles of potential scans were performed. And as a result of this study, it obtained the best possibility of VC devices in acidic medium, in the concentration of 2,5 x 10-3 mol L-1 and without number of 50 cycles of scans. To ensure the best conditions for immobilization of LVC-related biomolecules, thus providing the most suitable configuration for the construction and development of the biosensor.