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https://repositorio.ifgoiano.edu.br/handle/prefix/2389
Tipo: | Trabalho de Conclusão de Curso |
Título: | SHEDDING LIGHT ON THE TOXICITY OF SARS-CoV-2-DERIVED PEPTIDE IN NON-TARGET COVID-19 ORGANISMS: A STUDY INVOLVING INBRED AND OUTBRED MICE |
Autor(es): | Luz, Thiarlen Marinho da |
Primeiro Orientador: | Malafaia, Guilherme |
Primeiro Coorientador: | Araújo, Amanda Pereira da Costa |
Resumo: | Despite advances in research on the vaccine and therapeutic strategies of COVID-19, little attention has been paid to the possible (eco)toxicological impacts of the dispersion of SARS-CoV-2 particles in natural environments. Thus, in this study, we aimed to evaluate the behavioral and biochemical consequences of the short exposure of outbred and inbred mice (male Swiss and C57Bl/6J mice, respectively) to PSPD-2002 (peptide fragments of the Spike protein of SARS-CoV-2) synthesized in the laboratory. Our data demonstrated that after 24 h of intraperitoneal administration of PSPD-2002 (at 580 μg/kg) the animals did not present alterations in their locomotor, anxiolytic-like, or anxiety-like behavior (in the open field test), nor antidepressant-like or depressive behavior in the forced swimming test. However, the C57Bl/6J mice exposed to PSPD-2002 showed memory deficit in the novel object recognition task, which was associated with higher production of thiobarbituric acid reactive substances, as well as the increased suppression of acetylcholinesterase brain activity, compared to Swiss mice also exposed to peptide fragments. In Swiss mice the reduction in the activity of superoxide dismutase and catalase in the brain was not associated with increased oxidative stress biomarkers (hydrogen peroxide), suggesting that other antioxidant mechanisms may have been activated by exposure to PSPD-2002 to maintain the animals' brain redox homeostasis. Finally, the results of all biomarkers evaluated were applied into the "Integrated Biomarker Response Index" (IBRv2) and the principal component analysis (PCA), and greater sensitivity of C57Bl/6J mice to PSPD-2002 was revealed. Therefore, our study provides pioneering evidence of mammalian exposure-induced toxicity (non-target SARS-CoV-2 infection) to PSPD-2002, as well as “sheds light” on the influence of genetic profile on susceptibility/resistance to the effects of viral peptide fragments. |
Abstract: | Despite advances in research on the vaccine and therapeutic strategies of COVID-19, little attention has been paid to the possible (eco)toxicological impacts of the dispersion of SARS-CoV-2 particles in natural environments. Thus, in this study, we aimed to evaluate the behavioral and biochemical consequences of the short exposure of outbred and inbred mice (male Swiss and C57Bl/6J mice, respectively) to PSPD-2002 (peptide fragments of the Spike protein of SARS-CoV-2) synthesized in the laboratory. Our data demonstrated that after 24 h of intraperitoneal administration of PSPD-2002 (at 580 μg/kg) the animals did not present alterations in their locomotor, anxiolytic-like, or anxiety-like behavior (in the open field test), nor antidepressant-like or depressive behavior in the forced swimming test. However, the C57Bl/6J mice exposed to PSPD-2002 showed memory deficit in the novel object recognition task, which was associated with higher production of thiobarbituric acid reactive substances, as well as the increased suppression of acetylcholinesterase brain activity, compared to Swiss mice also exposed to peptide fragments. In Swiss mice the reduction in the activity of superoxide dismutase and catalase in the brain was not associated with increased oxidative stress biomarkers (hydrogen peroxide), suggesting that other antioxidant mechanisms may have been activated by exposure to PSPD-2002 to maintain the animals' brain redox homeostasis. Finally, the results of all biomarkers evaluated were applied into the "Integrated Biomarker Response Index" (IBRv2) and the principal component analysis (PCA), and greater sensitivity of C57Bl/6J mice to PSPD-2002 was revealed. Therefore, our study provides pioneering evidence of mammalian exposure-induced toxicity (non-target SARS-CoV-2 infection) to PSPD-2002, as well as “sheds light” on the influence of genetic profile on susceptibility/resistance to the effects of viral peptide fragments. |
Palavras-chave: | Environmental Toxicology Swiss mice C57Bl/6J mice proteins pandemic COVID-19 |
Área do CNPq: | CIENCIAS BIOLOGICAS |
Idioma: | por |
Pais: | Brasil |
Editor: | Instituto Federal Goiano |
Sigla da Instituição: | IF Goiano |
Campus: | Campus Urutaí |
Tipo de Acesso: | Acesso Aberto |
URI: | https://repositorio.ifgoiano.edu.br/handle/prefix/2389 |
Data do documento: | 16-Mar-2022 |
Aparece nas coleções: | Bacharelado em Ciências Biológicas |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Trabalho_de_curso_Thiarlen_Marinho_da_Luz.pdf | 1,89 MB | Adobe PDF | Visualizar/Abrir |
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