IDENTIFICAÇÃO E DESENVOLVIMENTO DE MARCADORES MICROSSATÉLITES CANDIDATOS PARA Brosimum gaudichaudii Trécul.: UMA ESPÉCIE MEDICINAL E NATIVA DO CERRADO

Abstract

The species Brosimum gaudichaudii belongs to the Moraceae family and is widely distributed in the Brazilian Cerrado. It is a fruitful and medicinal species, widely used in the treatment of diseases, not becoming a target of predatory extractivism. The lack and absence of conservation and management studies make it predatory, directly, by extractivism, thus increasing its natural population. to the lack of information on the population genetics of the species of microsatellite indicators configured as an efficient biotechnological tool and in evaluating the genetic flow, population and dispersion so that the management tools are adequate to be traced. Thus, the present study aims to promote the development of the first microsatellite markers for B. Gaudichaudii. For this, 27 accessions from a population of adults were used, using a single Mama-Cadela sample to support the DNA. With that, the DNA was extracted, quantified, digested with endonucleases and ligated to adapters; the fragments were pre-amplified via PCR and purified for selection of fragments of interest containing microsatellites by hybridization; the selected fragments were ligated to the cloning vector and by electroporation they were inserted into competent E. coli cells; finally, the transformed cells were half-pickled in solid LB for growth and the original clones were selected for construction of the genomic library. For supplies used, the software available on the internet was used free of charge. Thus, of which 78 clones sequenced in the round-trip direction from the genomic library48, a total of 96 sequences were produced, of which 78 were discarded in the editing process due to low quality, as from the 18 remaining sequences were found and 10 remaining sequences SSRs. Of the total of 10 sequences containing microsatellites, only six were suitable for the design of the primers. Therefore, the present study describes the first six microsatellite sites for the species in question.