Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.advisor1Pinto, Guilherme Malafaia-
dc.contributor.referee1Pinto, Guilherme Malafaia-
dc.contributor.referee2Menezes, Ivandilson Pessoa Pinto de-
dc.contributor.referee3Cunha, Ananda Helena Nunes-
dc.creatorSilva, Wellington Alves Mizael da-
dc.description.abstractEffluents are highly polluting waste, which can have negative impacts on people's health and the environment. However, studies investigating the effects of dermal exposure to these pollutants on the central nervous system of experimental mammalian models are still incipient. In this sense, and in order to expand the knowledge beyond the neurotoxic effects observed when the water contaminated by these xenobiotics is ingested, the neurobehavioral effects of the dermal exposure of males from the C57Bl/6J and Swiss lineages were analyzed. The animals were exposed the crude effluent (wet blue type) for two hours for five days, totaling 15 days of exposure. Afterwards, the animals were submitted to the high cross maze (anxiety predictive test) and to the object recognition test (used to identify memory deficit). Our data showed that dermal exposure to tannery effluent induced an anxiogenic behavior in the animals, when compared to those that did not have direct contact with these pollutants. It was also observed that the animals exposed to the effluent of tannery obtained lower rates of recognition of new objects, evidencing the memory deficiency and indicating a possible influence of the constituents of the tannery effluent on animal cognition. The present study supports the hypothesis that dermal exposure to tannery effluents causes behavioral disturbances in C57Bl/6J and Swiss micept_BR
dc.description.resumoOs efluentes do curtume constituem resíduos altamente poluidores, o que pode causar impactos negativos para a saúde das pessoas e para o ambiente. Contudo, estudos que investigaram os efeitos da exposição dérmica a estes poluentes no sistema nervoso central dos modelos experimentais de mamíferos são ainda incipientes. Nesse sentido, e com o objetivo de expandir o conhecimento além dos efeitos neurotóxicos observados quando a água contaminada por esses xenobióticos é ingerida, foram analisados os efeitos neurocomportamentais da exposição dérmica de machos de camundongos das linhagens C57Bl/6J e Swiss. Os animais foram expostos ao efluente de curtume bruto (tipo wet blue) durante duas horas, durante cinco dias, totalizando 15 dias de exposição. Depois, os animais foram submetidos ao labirinto em cruz elevado (teste preditivo da ansiedade) e ao teste de reconhecimento de objeto (utilizado na identificação de déficit de memória). Nossos dados mostraram que a exposição dérmica ao efluente de curtume induziu um comportamento ansiogênico nos animais, quando comparados àqueles que não tiveram contato direto com esses poluentes. Observou-se também que os animais expostos ao efluente de curtume obtiveram menores índices de reconhecimento de novos objetos, evidenciando assim deficiência de memória e indicando uma possível influência dos constituintes do efluente de curtume na cognição animal. O presente estudo atesta a hipótese de que a exposição dérmica a efluentes de curtumes provoca distúrbios comportamentais em camundongos C57Bl/6J e Swiss.pt_BR
dc.description.provenanceSubmitted by Patrícia Oliveira ( on 2019-01-31T17:34:04Z No. of bitstreams: 1 2018-07-18-11-12-20Dissertação_Mestrado_Welligton_ (1).pdf: 1810156 bytes, checksum: 5e4f68fcf01be7dedb94785bbaec2fce (MD5)en
dc.description.provenanceApproved for entry into archive by Patrícia Oliveira ( on 2019-01-31T18:04:17Z (GMT) No. of bitstreams: 1 2018-07-18-11-12-20Dissertação_Mestrado_Welligton_ (1).pdf: 1810156 bytes, checksum: 5e4f68fcf01be7dedb94785bbaec2fce (MD5)en
dc.description.provenanceApproved for entry into archive by Patrícia Oliveira ( on 2019-01-31T18:04:49Z (GMT) No. of bitstreams: 1 2018-07-18-11-12-20Dissertação_Mestrado_Welligton_ (1).pdf: 1810156 bytes, checksum: 5e4f68fcf01be7dedb94785bbaec2fce (MD5)en
dc.description.provenanceMade available in DSpace on 2019-01-31T18:04:49Z (GMT). No. of bitstreams: 1 2018-07-18-11-12-20Dissertação_Mestrado_Welligton_ (1).pdf: 1810156 bytes, checksum: 5e4f68fcf01be7dedb94785bbaec2fce (MD5) Previous issue date: 2018-03-13en
dc.publisherInstituto Federal Goianopt_BR
dc.publisher.departmentCampus Urutaípt_BR
dc.publisher.programPrograma de Pós-Graduação em Conservação de Recursos Naturais do Cerradopt_BR
dc.publisher.initialsIF Goianopt_BR
dc.relation.referencesAkkerman S, Blokland A, Reneerkens O, van Goethem NP, Bollen E, Gijselaers HJ, Lieben CK, Steinbusch HW, Prickaerts J. Object recognition testing: methodological considerations on exploration and discrimination measures. Behavioural Brain Research, 232(2): 335-347, 2012. 26 American Public Health Association (APHA). Standard methods for the examination of water and wastewater. 20. ed. New York: APHA, AWWA, WPCR, 1997. 1194 p. Bevins RA, Besheer J. Object recognition in rats and mice: a one-trial non-matching-tosample learning task to study ‘recognition memory’. Nature Protocols, 1: 1306-1311, 2006. Bolla KI. Neuropsychological assessment for detecting adverse effects of volatile organic compounds on the central nervous system. Environ Health Perspect. 1991 Nov; 95: 93– 98. Broadbent NJ, Gaskin S, Squire LR, Clark RE. Object recognition memory and the rodent hippocampus. Learning & Memory, 17: 5-11, 2010. Chandrasekaran V, Dilara K, Padmavathi R. Pulmonary functions in tannery workers – A cross sectional study. Indian Journal of Physiology and Pharmacology, 58: 206-210, 2014. Clarke JR, Cammarota M, Gruart A, Izquierdo I, Delgado-García JM. Plastic modifications induced by object recognition. PNAS, 107: 2652-2657, 2010. Costantini AS, Paci E, Miligi L, Buiatti E, Martelli C & Lenzi S. Cancer mortality among workers in the Tuscan tanning industry.British journal of industrial medicine, 46(6): 384-388, 1989. Cuberos E, Rodriguez AI, Pietro E. Niveles de cromo y alteraciones de salud en una población expuesta a las actividades de curtiembres en Bogotá, Colombia. Revista de Salud Pública, 11(2): 278-289, 2009. Ennaceur A, Delacour J. A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data. Behavioural Brain Research, 31: 47-59, 1988. Estrela DC, Silva WAM, Guimarães ATB, Mendes BO, Castro ALS, Torres ILS, Malafaia G. Predictive behaviors for anxiety and depression in female Wistar rats subjected to cafeteria diet and stress. Physiology & Behavior, 151: 252-263, 2015. Freitas TCM, Melnikov P. O uso e os impactos da reciclagem de cromo em indústrias de curtume em Mato Grosso do Sul, Brasil. Engenharia Sanitária e Ambiental, 2(4): 305- 310, 2006. 27 Gödecke MV, Rodrigues MAS, Naime RH. Resíduos de curtume: estudo das tendências de pesquisa. Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental, 7: 1357- 1378, 2012. Gordon CJ, Stead AG. Effect of nickel and cadmium chloride on autonomic and behavioral thermoregulation in mice. Neurotoxicology, 7: 97-106, 1986. Gowd SS & Govil PK. "Distribution of heavy metals in surface water of Ranipet industrial area in Tamil Nadu, India." Environmental monitoring and assessment 136 (1-3): 197- 207, 2008. Griebel G, Belzung C, Perrault G, Sanger DJ. Differences in anxiety-related behaviours and in sensitivity to diazepam in inbred and outbred strains of mice. Psychopharmacology, 148(2): 164-170, 2000. Guimarães ATB, Ferreira RO, Silva WAM, Castro ALS, Malafaia G. Parental exposure to tannery efluente cause ansiety-and-depression-like behaviors in mice offspring. JSM Anxiety Depress. 2016; 1: 1005. Haider S, Anis L, Batool Z, Sajid I, Naqvi F, Khaliq S, Ahmed S. Short term cadmium administration dose dependently elicits immediate biochemical, neurochemical and neurobehavioral dysfunction in male rats. Metab Brain Dis. 2015 Feb;30(1):83-92. doi: 10.1007/s11011-014-9578-4. Epub 2014 Jul 1. He MD, Xu SC, Zhang X, Wang Y, Xiong JC, Zhang X, Lu YH, Zhang L, Yu ZP, Zhou Z. Disturbance of aerobic metabolism accompanies neurobehavioral changes induced by nickel in mice. NeuroToxicology, 38: 9-16, 2013. Kalueff AV. Neurobiology of memory and axiety: from genes to behaviour. Neural Plasticiy, Article ID 78171: 12, 2007. Khan DA Mushtaq S, Khan FA, Khan MQ. Toxic effects of chromium on tannery workers at Sialkot (Pakistan). Toxicology and Industrial Health, 29:209-215, 2013. Komada M, Takao K, Miyakawa T. Elevated plus maze for mice. Journal Visualized Experiments, 22: 1-3, 2008. Liapi C, Zarros A, Theocharis S, Voumvourakis K, Anifantaki F, Gkrouzman E, et al.Short-term exposure to nickel alters the adult rat brain antioxidant status and 28 the activities of crucial membrane-bound enzymes: neuroprotection by L-cysteine. Biological Trace Element Research, 143: 1673-1681, 2011. Lofrano G, Meriç S, Zengin GE, Orhon D. Chemical and biological treatment technologies for leather tannery chemicals and wastewaters: a review. Science of the Total Environment, 461-462: 265-281, 2013. Marchetti C, Gavazzo P. Subunit-dependent effects of nickel on NMDA receptor channels. Molecular Brain Research, 117:139–44, 2003. Marsden CA, Bickerdike M, Cadogan AK, Wright I, Rex A, Fink H. "Serotonergic mechanisms and animal models of anxiety." colloques-institut national de la sante et de la recherche medicale colloques et seminaires: 75-75, 1993. Mathew SJ, Jeremy DC, Jack MG. "Neurobiological mechanisms of social anxiety disorder." American Journal of Psychiatry, 158: 1558-1567, 2014 Méndez-Armenta M, Ríos C. “Cadmium neurotoxicity,” Environmental Toxicology and Pharmacology, 23(3): 350–358, 2007. Miekisch W1, Schubert JK, Noeldge-Schomburg GF. Diagnostic potential of breath analysis-- focus on volatile organic compounds. Clin Chim Acta. 2004 Sep;347(1-2):25-39. Misra V, Pandey SD. Hazardous waste, impact on health and environment for development of better waste management strategies in future in India. Environ Int. 2005 Apr;31(3):417- 31. Moysés FS, Bertoldi K, Spindler C, Sanches EF, Elsner VR, Rodrigues MAS, Siqueira IR. Exposition to tannery wastewater did not alter behavioral and biochemical parameters in Wistar rats. Physiology & Behavior, 129:160-166, 2014. Moysés FS. Efeito da exposição ao efluente de curtume em diferentes modelos animais. (Tese de Doutorado). Universidade Federal do Rio Grande do Sul, Porto Alegre, 2014. Nutt, DJ. "Neurobiological mechanisms in generalized anxiety disorder. "The Journal of clinical psychiatry, 62: 22-7, 2000. 29 Otto DA, Hudnell HK, House DE, Mølhave L, Counts W. Exposure of humans to a volatile organic mixture. I. Behavioral assessment. Arch Environ Health. 1992 Jan- Feb;47(1):23-30. Paladini AC, Marder M, Viola H, Wolfman C, Wasowski C, Medina JH. Flavonoids and the central nervous system: from forgotten factors to potent anxiolytic compoundsJournal of Pharmacy and Pharmacology, 51(5): 519-26, 1999. Piéta-Dias C, De Lima MM, Presti TJ, Dornelles A, Garcia VA, Scalco FS, Schröder N. Memantine reduces oxidative damage and enhances long-term recognition memory in aged rats. Neuroscience, 146(4), 1719-1725, 2007. Rabelo LM, Silva BC, Almeida SF, Silva WAM, Mendes BO, Guimarães ATB, Silva AR, Castro ALS, Rodrigues ASL, Malafaia G. Memory deficit in Swiss mice exposed to tannery effluent. Neurotoxicology and Teratology, 55: 45-49, 2016. Rajagopal L, Massey BW, Huang M, Oyamada Y, Meltzer HY. The novel object recognition test in rodents in relation to cognitive impairment in Schizophrenia. Current Pharmaceutical Design, 20: 1-11, 2014. Rastogi SK, Amit P, Sachin T. "Occupational health risks among the workers employed in leather tanneries at Kanpur. ”Indian journal of occupational and environmental medicine 12(3): 132, 2008. Rodgers RJ, Dalvi A. Anxiety, defence and the elevated plus-maze. Neuroscience & Biobehavioral Reviews, 21: 801-810, 1997. Salazar EMG. Economia ecológica frente a economia industrial. El caso de la industria de la curtiduría em México. Argumentos, 21(56): 55-71, 2008. Saner G, Yüzbașiyan V, Çigdem S. Hair chromium concentration and chromium excretion in tannery workers. British Journal of Industrial Medicine, 41: 263-266,1984. Segura PA, François M, Gagnon C, Sauvé S. Review of the occurrence of anti-infectives in contaminated waste waters and natural and drinking water. Environmental Health Perspectives, 117: 675-684, 2009. 30 Shahzad K, Akhtar S, Mahmud S. Prevalence and determinants of asthma in adult male leather tannery workers in Karachi, Pakistan: a cross sectional study. BMC Public Health, 6(1), 292, 2006. Shakir L, Ejaz S, Ashraf M, Qureshi NA, Anjum AA, Iltaf I, Javeed A. Ecotoxicological risks associated with tannery effluent wastewater. Environmental Toxicology and Pharmacology, 34: 180-191, 2012. Singh R, Gautam N, Mishra A, Gupta R. Heavy metals and living systems: An overview. Indian J Pharmacol. 2011 May;43(3):246-53. doi: 10.4103/0253-7613.81505. Siqueira IR, Vanzella C, Bianchetti P, Rodrigues MAS, Stülp S. Anxiety-like behaviour in mice exposed to tannery wastewater: the effect of photoelectrooxidation treatment. Neurotoxicology and Teratology, 33: 481-484, 2011. Souza JM, Silva WAM, Mendes BO, Guimarães ATB, Almeida SF, Estrela DC, Silva AR, Rodrigues ASL, Malafaia G. Neurohehavioral evaluation of C57Bl/6J mice submitted to tannery effluents intake. JSM Anxiety Depress. 2016; 1: 1006. Veyalkin I, Gerein V.Retrospective cohort study of cancer mortality at the minsk leather tannery. Industrial Health, 44:69-74, 2006. Veyalkin IV, Alexander AM. "Proportionate cancer mortality among workers in the Belarussian tanning industry." American journal of industrial medicine 44 (6): 637-642, 2003. Zhang Q, Zhang L, Xiao X, Su Z, Zou P, Hu H, et al. Heavy metals chromium and neodymium reduced phosphorylation level of heat shock protein 27 in human keratinocytes. Toxico. In Vitro. 2010;24:1098–1104. [PubMed] Walf AA, Frye CA. The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nature Protocols, 2: 322-8, 2007. Wong C, O Carter S. "The GABAA Receptor: Subunit‐Dependent Functions and Absence Seizures." Epilepsy Currents. Sep; 1(1): 1–5, 2001. Yang XF, Fan GY, Liu DY, Zhang HT, Xu ZY, Ge YM, Wang ZL. Effect of cadmium exposure on the histopathology of cerebral cortex in juvenile mice. Biological Trace Element Research, 165: 167-172, 2015.pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectmamíferos - modelos experimentaispt_BR
dc.subjectefluentes agroindústriaispt_BR
dc.subjectrecursos hídricos - sustentabilidadept_BR
dc.subjectefluentes - poluição - contaminaçãopt_BR
dc.subjectcamundongos Swisspt_BR
dc.subjectáguas - poluentes - preservação ambientalpt_BR
dc.title.alternativeBehavioral Changes in Mice Exposed Dermically Tanning Effluentpt_BR
Appears in Collections:Mestrado Profissional em Conservação dos Recursos Naturais do Cerrado

Files in This Item:
File Description SizeFormat 
2018-07-18-11-12-20Dissertação_Mestrado_Welligton_ (1).pdfdissertação Wellington1,77 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.