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dc.contributor.advisor1Malafaia, Guilherme-
dc.contributor.advisor-co1Carvalho, Wanessa-
dc.contributor.advisor-co2Silva, Daniela-
dc.contributor.referee1Alves Júnior, José-
dc.contributor.referee2Silveira, Ricardo-
dc.creatorOliveira, José Silonardo-
dc.description.abstractThe damaging effects of the pesticides glyphosate (Gly), atrazine (Atra) and fipronil (Fip) are known in different organisms, however the mutagenic, genotoxic and morphotoxic potential in erythrocytes of freshwater Testudines is poorly studied. Thus, we aim to evaluate the toxicological potential of these compounds in puppies of Podocnemis expansa (Amazon turtles) hatched from eggs incubated artificially in substrate with different concentrations of the herbicides Gly and Atra and the insecticide Fip. The micronucleus test and other nuclear abnormalities, comet assay and morphometric measurements of the animals' circulating erythrocytes were used as biomarkers of toxicity. Puppies exposed to Gly (groups Gly-65 ppb and Gly-6500 ppb) had a higher frequency of erythrocytes with multilobulated nucleus, for groups Atra-2 ppb and Gly-65 ppb the notched and displaced nucleus abnormalities were the most frequent. In addition, it was observed that all treatments (Gly-6500 ppb, Atra-2 ppb, Atra-200 ppb, Fip-4 ppb and Fip-400 ppb), with the exception of the Gly-65 ppb group, induced a decrease in the erythrocyte area, increase in the “nuclear area: erythrocyte area” ratio and decrease in the circularity of erythrocytes and their nuclei, which indicates a clear effect on the size and shape of these cells. On the other hand, in the comet assay there was no evidence suggestive of the genotoxic effect of pesticides. In conclusion, this study pioneered the mutagenic, genotoxic and morphotoxic potential of pesticides in puppies of P. expansa exposed in ovo to Gly, Atra and Fip, which constitutes how these compounds can affect the health of these animals.pt_BR
dc.description.resumoOs efeitos danosos dos agrotóxicos glifosato (Gly), atrazina (Atra) e fipronil (Fip) são conhecidos em diferentes organismos, porém os potenciais mutagênico, genotóxico e morfotóxico em eritrócitos de Testudines de água doce é pouco estudado. Assim, objetivou-se avaliar os potenciais toxicológicos desses compostos em recém-eclodidos de Podocnemis expansa (tartarugas-da-amazônia) nascidos de ovos incubados artificialmente em substrato com diferentes concentrações dos herbicidas Gly e Atra e do inseticida Fip. O teste do micronúcleo e outras anormalidades nucleares, ensaio cometa e medidas morfométricas de eritrócitos circulantes dos animais foram utilizados como biomarcadores de toxicidade. Os recém-eclodidos expostos aos grupos Gly-65 ppb e Gly-6500 ppb apresentaram maior frequência de eritrócitos com núcleo multilobulado. Para os grupos Atra-2 ppb e Gly-65 ppb as anormalidades núcleo entalhado e núcleo deslocado foram as mais frequentes. Além disso, observou-se que em Gly-6500 ppb, Atra-2 ppb, Atra-200 ppb, Fip-4 ppb e Fip-400 ppb, com exceção do grupo Gly-65 ppb, induziram diminuição da área eritrocitária, aumento da relação “área nuclear: área eritrocitária” e diminuição da circularidade dos eritrócitos e seus núcleos, o que indica claro efeito sobre o tamanho e forma dessas células. Por outro lado, no ensaio cometa não se observou evidências sugestivas de efeito genotóxico dos pesticidas. No entanto, concluiu-se que as concentrações de glifosato, atrazina e fripronil permitidas pela legislação ambiental brasileira causam morfotoxidade eritrocitária e efeito aneuploidogênico, sem efeito genotoxico identificado pelo ensaio cometa. A ausência de efeitos genotóxicos identificados pelo ensaio cometa possivelmente é explicada pela longa exposição aos agrotóxicos. Os longos períodos de exposição podem elevar os danos genotóxicos, causando morte celular. Assim como muitos outros, este estudo comprova a ação danosa do glifosato, atrazina e fipronil à biota, contudo, além destes, diversos pesticidas são prejudiciais, muitos dos quais se tem pouco ou nenhum conhecimento dos impactos lesivos ao meio ambiente, e a própria vida humana, a longo prazo.pt_BR
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dc.publisherInstituto Federal Goianopt_BR
dc.publisher.departmentCampus Urutaípt_BR
dc.publisher.programPrograma de Pós-Graduação em Conservação de Recursos Naturais do Cerradopt_BR
dc.publisher.initialsIF Goianopt_BR
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dc.rightsAcesso Abertopt_BR
dc.subjectAgrotóxicos, genotoxicidade, morfotoxicidade, mutagenicidade, pesticidas, toxicologia.pt_BR
dc.subjectTestudines, pesticides, toxicology, mutagenicity, genotoxicity, morphotoxicity.pt_BR
dc.title.alternativeTOXICOLOGICAL EFFECTS OF PESTICIDE CONTAMINATION IN NEWLY HATCHED OF Podocnemis expansa (Testudines: Pocnemididae)pt_BR
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