Aplicação de qualidades de luz leds no desempenho morfofisiológico e bioquímico de plântulas de Brosimum gaudichaudii tréc. in vitro

Abstract

Belonging to the family Moraceae the species Brosimum gaudichaudii Tréc. has furanocoumarins with therapeutic potential in the vitiligo treatment. The metabolites baptapene and psoralen present mainly in the roots of this species stand out for their melanocytic effect in combating vitiligo. In this context, the objective was to evaluate and enhance the growth, physiological, anatomical and biochemical characteristics of Brosimum gaudichaudii Tréc. in vitro under different light qualities. Light-emitting diodes (LEDs) were used in the wavelengths white (400-700nm), blue (400-490 nm), red (600-700 nm) and different lighting combinations with blue (A) and red LEDs (V): A / V 1: 1 and A / V 3: 1. Nodal segments were cultured in 50 mL tubes with 100% MS medium, containing agar, with the addition of 30 μM benzylaminopurine (BAP), 3% saccharose, under a 16h photoperiod. After 50 days of treatment imposition, the seedlings were submitted to biometric, pigment, photosynthetic efficiency, anatomical, histochemical and chromatographic analyzes. It was found that the blue light and A/V 3:1 proportion are more satisfactory for bergaptene production, better photosynthetic rate and anthocinins content, the quality of A/V 1:1 light influenced only the psoralen production. For obtaining both metabolites and obtaining plants with better growth, the A/V 3:1 light is indicated.