PERFOMANCE DE DOADORAS Senepol APÓS Opu e Civ: TAXAS DE RECUPERAÇÃO DE OÓCITOS

Abstract

Senepol is a 100% taurine breed, intended for cutting. With the objective of establishing donors with high reproductive efficiency and advancing the production of animals with high genetics, we are seeking more and more biotechnologies that can accelerate this process. This study aimed to evaluate the performance of Senepol donors after OPU and IVC by oocyte recovery rates. A data collection of ultrasonically guided follicular aspiration and in vitro oocyte culture data from Senepol breeders was carried out during the year 2013. Only data from donors submitted to ten or more aspirations in that year were considered for this study. During the months of 2013, Samvet embryo laboratory data were collected, including: number of total oocytes after aspiration, number of viable and non-viable oocytes after aspiration, oocyte number of degrees I, II, III and% of oocytes viable oocytes after in vitro culture. The descriptive statistics for the characteristics analyzed and evaluation of the significance of the effects of the months of the year and individual factor of the donor were performed by Analysis of Variance and later Tukey's test determined through the software SAS®, assuming a level of statistical significance of 5 %. The mean values of 27.13 ± 14.38 total oocytes were observed after donor per donor; 3.69 ± 2.28 non-viable oocytes; 23.17 ± 12.94 viable oocytes; 2.07 ± 2.76 grade I oocytes; 10.13 ± 6.12 grade II oocytes; 10.61 ± 7.76 grade III oocytes; 23.24 ± 13.14% of viable oocytes after IVC. The mean 27.13 total oocytes of the Senepol breed of this study is considered satisfactory. The influence of the effects of the months of the year and individual factor of the donors on the characteristics analyzed was significant (p> 0.05). It is concluded that the recovery rates of oocytes from Senepol donors showed satisfactory results, with significant variation between donors and between months of the year. The months of February, March and April were the ones with the best rates.