https://repositorio.ifgoiano.edu.br/handle/prefix/140 Mon, 11 May 2026 12:44:39 GMT 2026-05-11T12:44:39Z http://repositorio.ifgoiano.edu.br:80/retrieve/213/194.png https://repositorio.ifgoiano.edu.br/handle/prefix/140 https://repositorio.ifgoiano.edu.br/handle/prefix/6510 Título: CONVERGENT GALL STRUCTURES INDUCED BY A SINGLE INSECT SPECIES ACROSS MULTIPLE PLANT BODY PARTS OF Sapium glandulosum (L.) Morong (L.) (Euphorbiaceae) Autor(es): Wickert, Daniela Maria Primeiro Orientador: Kuster, Vinicius Coelho Primeiro Membro da Banca: Oliveira, Denis Coelho de Segundo Membro da Banca: Costa, Elaine Cotrim Abstract: Galls are anomalous structures formed in plants in response to induction by insects, mites, or other organisms. In reproductive organs, they generally develop from ovules, often resulting in the formation of false fruits, whereas galls in fruits are rarely documented. This study investigates the galls induced by Neolithus fasciatus Scott, 1882 (Hemiptera) on Sapium glandulosum (L.) Morong, both in the floral nectary and in the fruit pericarp, with emphasis on the anatomy and pectic composition of the cell walls. Anatomical analysis was performed using historesin embedding and sectioning with a rotary microtome, whereas immunocytochemistry was used to assess homogalacturonans (HGs) and rhamnogalacturonans I (RG-I) in the galls and their corresponding ungalled organs. Inflorescence galls originate from the cellular redifferentiation of the floral nectary, which loses its sugar-secreting and pollinator-attracting functions and assumes a new structural identity aimed at protecting the gall. In contrast, pericarp galls form from the exocarp and mesocarp, primarily from the parenchyma. Structurally, both the floral nectary galls and the pericarp galls are similar, exhibiting a uniseriate epidermis with a thin cuticle and a cortex composed externally of chlorophyllous parenchyma and internally of fundamental parenchyma, as well as collateral vascular bundles. Immunocytochemical analysis revealed strong labeling of methyl-esterified HGs in the similar galls induced in different organs, indicating the maintenance of elasticity and active growth.; Pericarp galls also showed marked labeling for RG-I with (1→4)-β-D-galactan side chains, which are associated with cell hypertrophy and plasticity. These pectins were also intensely labeled in the phloem cell walls, suggesting increased flexibility and support for the elevated flow of photoassimilates required to sustain the inducing insect, which has a sucking feeding apparatus. The maintenance of a complex pectic matrix, distinct among host organs, reveals the influence of the host tissue’s organization, whereas the structural similarity of the gall tissues highlights the central role of the inducer in phenotypic convergence. Editor: Instituto Federal Goiano Tipo: Dissertação Thu, 30 Oct 2025 00:00:00 GMT https://repositorio.ifgoiano.edu.br/handle/prefix/6510 2025-10-30T00:00:00Z https://repositorio.ifgoiano.edu.br/handle/prefix/6085 Título: ANÁLISE CITOGENÉTICA DE CINCO ESPÉCIES DO GÊNERO Boana (anura: hylidae) E DUAS ESPÉCIES DO GÊNERO Rhinella (anura: bufonidae) Autor(es): Silva, Ana Caroline Cândida da Primeiro Orientador: Mendonça, Maria Andréia Corrêa Abstract: Brazil hosts the greatest diversity of amphibians in the world, with the order Anura, which includes toads, frogs, and tree frogs, standing out. Despite this richness, the genetic aspect of these animals has been poorly explored, especially in relation to karyotypic characterization. Cytogenetics has been an important tool for complementing data that aids in the identification and taxonomic classification of anurans, enabling the detection of chromosomal variations and understanding the evolution of the group. However, challenges remain, especially concerning species that have not yet been karyotyped. In this context, a scientometric study about the cytogenetics of anurans in Brazil was carried out, using the platforms "Web of Science," "Scielo," and "Scopus," with keywords related to karyotypes and chromosomes. There were found 163 articles about 470 specimens, revealing regional disparities in the research, with the Atlantic Forest being the most sampled biome, while biomes such as the Pantanal, Caatinga, and Pampa show significant gaps. Furthermore, based on the results obtained from the scientometric approach, species with potential for karyotypic characterization studies were identified. Therefore, seven species were analyzed and karyotyped, five of which belong to the genus Boana (B. albopunctata, B. crepitans, B. multifasciata, B. paranaiba, B. raniceps), and two to the genus Rhinella (R. diptycha and R. mirandaribeiroi). The karyotypes of Boana paranaiba and Rhinella mirandaribeiroi were described for the first time in this study, both showing 2n = 2x = 22 chromosomes, like the other species analyzed, except for B. crepitans and B. raniceps, which had a chromosomal number of 2n = 2x = 24. It was also identified the presence of B chromosomes in Boana albopunctata. Therefore, continuing cytogenetic studies, especially in underexplored biomes, is crucial to fill knowledge gaps, address taxonomic issues, and support conservation strategies. Given the growing environmental threats, advancements in this area are essential to prevent the loss of species before they are even cytogenetically described. Editor: Instituto Federal Goiano Tipo: Dissertação Tue, 25 Mar 2025 00:00:00 GMT https://repositorio.ifgoiano.edu.br/handle/prefix/6085 2025-03-25T00:00:00Z https://repositorio.ifgoiano.edu.br/handle/prefix/5983 Título: ANÁLISE GENOTÓXICA E MUTAGÊNICA EM INSETOS, COM ÊNFASE NA CIGARRA Fidicina toulgoeti BOULARD & MARTINELLI, 1996 (hemiptera: auchenorrhyncha) Autor(es): Barros, Jhonatan Figueiredo de Primeiro Orientador: Santos, Lia Raquel de Souza Abstract: This dissertation addresses genotoxicity and mutagenicity in insects, with emphasis on the cicada Fidicina toulgoeti (Hemiptera: Auchenorrhyncha), in addition to conducting a systematic review and experimental ecotoxicological analyses. The first chapter consisted of a scientometric review on the use of the comet assay and micronucleus test in insects, based on the Web of Science database, covering articles published between 2004 and 2025. A total of 34 studies from nine countries were identified, with India, Turkey, and Egypt standing out. The orders Lepidoptera and Diptera were the most represented, and metals such as zinc, copper, and lead were among the main xenobiotics evaluated. The review highlighted the growing relevance of these biomarkers for insect ecotoxicology and pointed out geographic and thematic gaps in the literature. The second chapter involved the application of these biomarkers to hemocytes of F. toulgoeti collected in the Cerrado biome of Goiás, Brazil, in order to verify genotoxic damage through the micronucleus test and comet assay. The analyses revealed the presence of cellular anomalies and DNA damage, showing a positive trend between the frequency of micronuclei and comet assay parameters, although without significant statistical correlation. The results reinforce the potential of Hemiptera as bioindicators and the importance of the integrated use of these assays to assess environmental impacts on native Cerrado insects. Editor: Instituto Federal Goiano Tipo: Trabalho de Conclusão de Curso Wed, 29 Oct 2025 00:00:00 GMT https://repositorio.ifgoiano.edu.br/handle/prefix/5983 2025-10-29T00:00:00Z https://repositorio.ifgoiano.edu.br/handle/prefix/5844 Título: IDENTIFICAÇÃO DE ABELHAS NATIVAS NO CERRADO COM O USO DE INTELIGÊNCIA ARTIFICIAL Autor(es): França, Elzi Leida do Carmo Primeiro Orientador: Farache, Fernando Henrique Antoniolli Primeiro Membro da Banca: Farache, Fernando Henrique Antoniolli Segundo Membro da Banca: Costa, Adriano Ferraz da Terceiro Membro da Banca: Menino, Gisele Cristina de Oliveira Abstract: This study proposed and evaluated the use of convolutional neural networks, through the YOLO architecture, for automatic identification of native Cerrado bees from images, contributing to biodiversity conservation and to the advancement of tools supporting pollinator monitoring. Images obtained from different sources and properly labeled were used, covering several native species. In the general experiment, the model achieved significant metrics, with strong precision and recall rates for most species, demonstrating its efficiency in detection and classification. Additionally, a second experiment was carried out focusing on the morphologically similar species Trigona hyalinata (Guaxupé) and Trigona spinipes (Irapuã), in which the model achieved an overall accuracy of 79.67%, a precision of 79.35% for Guaxupé, and 80.00% for Irapuã, confirming its discrimination capability even in scenarios of high visual similarity. The results demonstrate the feasibility and robustness of the approach, highlighting its potential for application in monitoring programs, sustainable management, and conservation of native bees in the Cerrado. Editor: Instituto Federal Goiano Tipo: Dissertação Wed, 27 Aug 2025 00:00:00 GMT https://repositorio.ifgoiano.edu.br/handle/prefix/5844 2025-08-27T00:00:00Z