https://repositorio.ifgoiano.edu.br/handle/prefix/170 2026-07-21T03:23:49Z https://repositorio.ifgoiano.edu.br/handle/prefix/6817 Título: O PAPEL DE DIFERENTES FIXADORES NA IDENTIFICAÇÃO DA COMPOSIÇÃO DA SECREÇÃO DE COLÉTERES Autor(es): Silveira, Amanda Santos Primeiro Orientador: Dalvi, Valdnéa Casagrande Primeiro Membro da Banca: Vasconcelos, Jaqueline Martins Segundo Membro da Banca: Vasconcelos Filho, Sebastião Carvalho Abstract: Fixation is a fundamental step in plant anatomy, with its main function being to halt cellular metabolism and preserve the structural integrity and chemical composition of tissues as close as possible to their in vivo state. The choice of fixative agent is a determining factor in sample preparation, as different solutions interact differently with biomolecules, potentially affecting the retention of specific metabolites and the formation of artifacts. In this context, this project analyzes whether different histological fixatives interfere with the results of histochemical tests in the colleters of Genipa americana (Rubiaceae). The study compares the efficacy of two fixatives — FAA50 and Buffered Neutral Formalin (BNF) — in preserving the structural and histochemical composition of colleter secretions. The plant samples were collected, fixed, dehydrated, embedded in resin, and subjected to histochemical tests for the identification of polysaccharides, proteins, and phenolic compounds. The slides were mounted, analyzed, and photographed. The colleters of G. americana occur on the inner surface of the stipules and are of the standard type. Neutral polysaccharides, pectins, acidic mucopolysaccharides, and proteins were observed in the secretion. We found that the choice of fixative affects the preservation of cellular structures and the staining of histochemical test results. The results also demonstrated that FNT performed better in cell preservation, while the FAA stood out for enhancing staining. Thus, this work enriches the field of plant anatomy by showing that the type of fixative should be a strategic choice aligned with the study's requirements. Editor: Instituto Federal Goiano Tipo: Trabalho de Conclusão de Curso 2026-06-22T00:00:00Z https://repositorio.ifgoiano.edu.br/handle/prefix/6782 Título: ALTERAÇÕES ANATÔMICAS EM FOLHAS DE GIRASSOL (Helianthus annus L.) CULTIVADOS EM SOLO CONTAMINADO COM CÁDMIO Autor(es): Silveira, Gianieily Alves Flausino de Queiroz Primeiro Orientador: Dalvi, Valdnéa Casagrande Primeiro Membro da Banca: Vasconcelos Filho, Sebastião Carvalho Segundo Membro da Banca: Mendonça, Maria Andréia Corrêa Abstract: Soil contamination by toxic elements represents an increasing environmental concern, with cadmium (Cd) standing out due to its high toxicity, elevated mobility, and ease of uptake by plants. This metal is introduced into the environment mainly through anthropogenic activities, such as the use of phosphate fertilizers and the improper disposal of waste, where it can bioaccumulate within the food chain and pose environmental and human health risks even at low concentrations. In plants, Cd induces morphological, physiological, and anatomical alterations, including impairment of photosynthesis, transpiration, stomatal function, and, consequently, plant growth and development. Although several studies have investigated the morphophysiological effects of Cd on sunflower (Helianthus annuus L.), information regarding the anatomical responses of this species to Cd contamination remains limited. Therefore, the present study aimed to evaluate the anatomical changes in sunflower leaves exposed to different Cd concentrations. The experiment was conducted under greenhouse conditions using three Cd concentrations (2.5, 5.0, and 7.5 mg dm⁻³), in addition to a control treatment, applied as CdCl₂, with five replicates per treatment. Sixty days after germination, samples from the median region of fully expanded leaves were collected, fixed, and processed for microscopic analyses. Control plants exhibited the typical leaf structure, consisting of a uniseriate epidermis with stomata and trichomes on both leaf surfaces, a dorsiventral mesophyll, and vascular bundles. Exposure to Cd resulted in progressive, concentration dependent anatomical alterations, including epidermal collapse, elongation and disorganization of mesophyll cells, enlargement of intercellular spaces, and severe structural deformation at the highest Cd concentrations. It is concluded that Cd causes significant anatomical damage to sunflower leaves, even at relatively low concentrations, demonstrating the high structural sensitivity of this species to Cd exposure. Furthermore, the findings highlight the importance of plant anatomy as a bioindicator tool for assessing the ecotoxicity of potentially toxic elements, including Cd. Editor: Instituto Federal Goiano Tipo: Trabalho de Conclusão de Curso 2026-06-26T00:00:00Z https://repositorio.ifgoiano.edu.br/handle/prefix/6766 Título: SEBASTIÃO JOSÉ DE OLIVEIRA: TRAJETÓRIA ACADÊMICA DE UM BIÓLOGO NO CONTEXTO DA SUB-REPRESENTAÇÃO NEGRA NA CIÊNCIA BRASILEIRA Autor(es): Oliveira, Wender Pereira de Primeiro Orientador: Ribeiro, Cristiane Maria Primeiro Membro da Banca: Silva, Rubislei Sabino da Segundo Membro da Banca: Pego, Douglas Souza Terceiro Membro da Banca: Ribeiro, Cristiane Maria Abstract: The underrepresentation of Black people in Brazilian science reveals the enduring effects of structural racism and the erasure of Black intellectuals. In this context, this study aims to analyze the academic and scientific trajectory of the Black biologist Sebastião José de Oliveira, highlighting his relevance to entomology and to Black representation in science. The methodology consisted of qualitative, bibliographic, and documentary research, based on the analysis of scientific articles, historical works, and institutional documents. The study discusses his educational background, pioneering contributions to entomology, and the impacts of structural racism and epistemicide on the exclusion of Black scientists. Furthermore, it highlights his trajectory as a form of resistance against academic whiteness and historical erasure, reinforcing the need for a more plural and inclusive scientific community. It is concluded that his trajectory represents not only a scientific achievement but also a milestone in the struggle for the recognition of Black scientists in the history of Brazilian science, whose legacy deserves to be recovered and disseminated. Editor: Instituto Federal Goiano Tipo: Trabalho de Conclusão de Curso 2026-06-19T00:00:00Z https://repositorio.ifgoiano.edu.br/handle/prefix/6739 Título: CONTRIBUIÇÕES DO ENSINO DE BIOLOGIA PARA A EDUCAÇÃO SEXUAL NO ENSINO MÉDIO Autor(es): Nacimento, Marco Aurélio Barbosa Primeiro Orientador: Firmino, Simone Gomes Abstract: This Final Course Project addresses the importance of sex education in high school, with an emphasis on the contributions of biology teaching to the comprehensive education of students. The study seeks to understand how the scientific and pedagogical approach to sexuality, mediated by biological content, influences the development of responsible, conscious, and respectful attitudes among adolescents. The research is qualitative in nature, based on a literature review and critical analysis of authors who discuss the role of schools and biology teachers in promoting sex education. It starts from the hypothesis that the absence of a systematic and continuous approach favors the perpetuation of taboos, prejudices, and misinformation. The results indicate that the inclusion of sex education in the curriculum, linked to biology content and developed in an ethical and interdisciplinary manner, contributes to strengthening self-esteem, preventing sexually transmitted infections, respecting diversity, and building critical thinking. It is concluded that biology teaching plays a fundamental role in promoting sex education, being an essential element for the integral development of students and for the formation of more informed and conscious citizens. Editor: Instituto Federal Goiano Tipo: Trabalho de Conclusão de Curso 2026-06-16T00:00:00Z