https://repositorio.ifgoiano.edu.br/handle/prefix/140 2026-03-15T11:17:33Z 2026-03-15T11:17:33Z Silva, Ana Caroline Cândida da https://repositorio.ifgoiano.edu.br/handle/prefix/6085 2026-01-06T14:23:33Z 2025-03-25T00:00:00Z

Título: ANÁLISE CITOGENÉTICA DE CINCO ESPÉCIES DO GÊNERO Boana (anura: hylidae) E DUAS ESPÉCIES DO GÊNERO Rhinella (anura: bufonidae) Autor(es): Silva, Ana Caroline Cândida da Primeiro Orientador: Mendonça, Maria Andréia Corrêa Abstract: Brazil hosts the greatest diversity of amphibians in the world, with the order Anura, which includes toads, frogs, and tree frogs, standing out. Despite this richness, the genetic aspect of these animals has been poorly explored, especially in relation to karyotypic characterization. Cytogenetics has been an important tool for complementing data that aids in the identification and taxonomic classification of anurans, enabling the detection of chromosomal variations and understanding the evolution of the group. However, challenges remain, especially concerning species that have not yet been karyotyped. In this context, a scientometric study about the cytogenetics of anurans in Brazil was carried out, using the platforms "Web of Science," "Scielo," and "Scopus," with keywords related to karyotypes and chromosomes. There were found 163 articles about 470 specimens, revealing regional disparities in the research, with the Atlantic Forest being the most sampled biome, while biomes such as the Pantanal, Caatinga, and Pampa show significant gaps. Furthermore, based on the results obtained from the scientometric approach, species with potential for karyotypic characterization studies were identified. Therefore, seven species were analyzed and karyotyped, five of which belong to the genus Boana (B. albopunctata, B. crepitans, B. multifasciata, B. paranaiba, B. raniceps), and two to the genus Rhinella (R. diptycha and R. mirandaribeiroi). The karyotypes of Boana paranaiba and Rhinella mirandaribeiroi were described for the first time in this study, both showing 2n = 2x = 22 chromosomes, like the other species analyzed, except for B. crepitans and B. raniceps, which had a chromosomal number of 2n = 2x = 24. It was also identified the presence of B chromosomes in Boana albopunctata. Therefore, continuing cytogenetic studies, especially in underexplored biomes, is crucial to fill knowledge gaps, address taxonomic issues, and support conservation strategies. Given the growing environmental threats, advancements in this area are essential to prevent the loss of species before they are even cytogenetically described. Editor: Instituto Federal Goiano Tipo: Dissertação

2025-03-25T00:00:00Z Barros, Jhonatan Figueiredo de https://repositorio.ifgoiano.edu.br/handle/prefix/5983 2025-12-10T23:48:10Z 2025-10-29T00:00:00Z

Título: ANÁLISE GENOTÓXICA E MUTAGÊNICA EM INSETOS, COM ÊNFASE NA CIGARRA Fidicina toulgoeti BOULARD & MARTINELLI, 1996 (hemiptera: auchenorrhyncha) Autor(es): Barros, Jhonatan Figueiredo de Primeiro Orientador: Santos, Lia Raquel de Souza Abstract: This dissertation addresses genotoxicity and mutagenicity in insects, with emphasis on the cicada Fidicina toulgoeti (Hemiptera: Auchenorrhyncha), in addition to conducting a systematic review and experimental ecotoxicological analyses. The first chapter consisted of a scientometric review on the use of the comet assay and micronucleus test in insects, based on the Web of Science database, covering articles published between 2004 and 2025. A total of 34 studies from nine countries were identified, with India, Turkey, and Egypt standing out. The orders Lepidoptera and Diptera were the most represented, and metals such as zinc, copper, and lead were among the main xenobiotics evaluated. The review highlighted the growing relevance of these biomarkers for insect ecotoxicology and pointed out geographic and thematic gaps in the literature. The second chapter involved the application of these biomarkers to hemocytes of F. toulgoeti collected in the Cerrado biome of Goiás, Brazil, in order to verify genotoxic damage through the micronucleus test and comet assay. The analyses revealed the presence of cellular anomalies and DNA damage, showing a positive trend between the frequency of micronuclei and comet assay parameters, although without significant statistical correlation. The results reinforce the potential of Hemiptera as bioindicators and the importance of the integrated use of these assays to assess environmental impacts on native Cerrado insects. Editor: Instituto Federal Goiano Tipo: Trabalho de Conclusão de Curso

2025-10-29T00:00:00Z França, Elzi Leida do Carmo https://repositorio.ifgoiano.edu.br/handle/prefix/5844 2025-11-03T21:57:40Z 2025-08-27T00:00:00Z

Título: IDENTIFICAÇÃO DE ABELHAS NATIVAS NO CERRADO COM O USO DE INTELIGÊNCIA ARTIFICIAL Autor(es): França, Elzi Leida do Carmo Primeiro Orientador: Farache, Fernando Henrique Antoniolli Primeiro Membro da Banca: Farache, Fernando Henrique Antoniolli Segundo Membro da Banca: Costa, Adriano Ferraz da Terceiro Membro da Banca: Menino, Gisele Cristina de Oliveira Abstract: This study proposed and evaluated the use of convolutional neural networks, through the YOLO architecture, for automatic identification of native Cerrado bees from images, contributing to biodiversity conservation and to the advancement of tools supporting pollinator monitoring. Images obtained from different sources and properly labeled were used, covering several native species. In the general experiment, the model achieved significant metrics, with strong precision and recall rates for most species, demonstrating its efficiency in detection and classification. Additionally, a second experiment was carried out focusing on the morphologically similar species Trigona hyalinata (Guaxupé) and Trigona spinipes (Irapuã), in which the model achieved an overall accuracy of 79.67%, a precision of 79.35% for Guaxupé, and 80.00% for Irapuã, confirming its discrimination capability even in scenarios of high visual similarity. The results demonstrate the feasibility and robustness of the approach, highlighting its potential for application in monitoring programs, sustainable management, and conservation of native bees in the Cerrado. Editor: Instituto Federal Goiano Tipo: Dissertação

2025-08-27T00:00:00Z Pereira, Juliane de Souza https://repositorio.ifgoiano.edu.br/handle/prefix/5579 2025-07-10T18:54:10Z 2025-05-09T00:00:00Z

Título: MORFOANATOMIA DAS MARGENS FOLIARES EM ESPÉCIES DE CELASTRACEAE NEOTROPICAIS: UMA ASSOCIAÇÃO COM GLÂNDULAS? Autor(es): Pereira, Juliane de Souza Primeiro Orientador: Dalvi, Valdnéa Casagrande Abstract: Celastraceae, a family within the order Celastrales, presents a complex taxonomy, with recent reclassifications currently recognizing 13 subfamilies. The family exhibits a wide variety of leaf margin types, including entire, crenate, serrate, spiny, dentate, spiny-dentate, and glandular-dentate forms. This morphological trait, particularly the presence of glands, has been used by taxonomists to delimit taxa at various hierarchical levels. Colleters associated with leaf margins have so far been reported in only two species of the family. In this context, the present study aimed to investigate the presence of glands associated with different types of leaf margins in Neotropical species of Celastraceae, through morphoanatomical and micromorphological analyses, and to confirm the identity of these glands. A total of 21 species belonging to 12 genera and four Neotropical subfamilies of Celastraceae (Celastroideae, Salacioideae, Hippocrateoideae, and Maytenoideae) were sampled. Young and mature leaves were obtained from herbarium specimens and/or field collections in areas in the Cerrado and Atlantic Forest, and processed using standard techniques. Four types of leaf margins were observed: eight species with entire margins, ten with crenate, one with serrate, and three with spinescent margins. Leaf venation analysis revealed a predominance (71.43%) of the semicraspedodromous pattern, with marginal endings varying between looped, pointed, incomplete, or absent. Glands were found in 18 of the 21 species, being absent in those with spinescent margins (Monteverdia aquifolium and M. ilicifolia) and in Schaefferia argentinensis, which has an entire margin. These glands position, their secretory activity, anatomical structure, lack of vascularization, and secretion composition allowed their identification as colleters. These colleters are of the standard type, producing a polysaccharide-rich mucilaginous secretion. They are deciduous in mature leaves, leaving scars. The highest colleters density was observed in the subfamily Maytenoideae. The presence of colleters on the leaf margins of Neotropical species of Celastraceae proved to be a common feature, reinforcing the relevance of this secretory structure to the taxonomy of the family, as it may serve as a stable and distinctive morphological character useful for the delimitation of taxonomic groups. Editor: Instituto Federal Goiano Tipo: Dissertação

2025-05-09T00:00:00Z