ANÁLISE QUIMIOTAXONÔMICA DE VARIEDADES DE HANCORNIA SPECIOSA ATRAVÉS DA TÉCNICA DE RMN HR-MAS E QUIMIOMETRIA

Abstract

The objective of this study is the chemotaxonomic evaluation of Hancornia speciosa varieties and its differentiation by High Resolution Magic Angle Spinning Nuclear Magnetic Resonance (1H HR-MAS-NMR) and chemometric techniques. Leaves were used from 70 trees produced in a germoplasm bank comprises by gardneri, speciosa, cuyabensis and pubescens varieties from the states of Goiás, Mato Grosso, Mato Grosso do Sul, Tocantins, Bahia and Minas Gerais. In a rotor with 50 uL, 12 mg of sample sprayed with 40 µL of deuterated methanol were packed. After packaging and until the start of the acquisition, a time of one hour was expected to waterlogging the solvent in the sample. The spectra were obtained in triplicate, all analysis parameters were optimized, and the results were converted to the construction of the data matrix. It was held on principal component analysis (PCA) to discriminate samples and hierarchical cluster analysis (HCA) to display the degree of similarity. The results were evaluated with selection of variables and without this selection, focusing on medium or autoscaled and the similarity as variety, location and family. The variety speciosa groups in relation to others with esters difference in the chemical composition of the leaves. In gardneri variety, it was observed variations in sign aldehydes and corresponding clusters to collection sites. The variety pubescens showed distinct clusters to speciosa, while cuyabensis presented groupings in all variables.